Electron transfer proteins
In many metabolic reactions, a protein that acts as an electron carrier binds to an enzyme that acts as its reductase. After it receives an electron, it dissociates and then binds to the next … 展开

The molecular structures of many protein complexes have been unlocked by the technique of X-ray crystallography. The first structure to be solved … 展开

• Protein concentration, which in turn are affected by expression levels and degradation rates;
• Protein affinity for proteins or other binding ligands; 展开

Computational prediction of protein–protein interactions
The experimental detection and characterization of PPIs is … 展开

To describe the types of protein–protein interactions (PPIs) it is important to consider that proteins can interact in a "transient" way (to produce some specific effect in a short time, like signal transduction) or to interact with other proteins in a "stable" … 展开

The study of the molecular structure can give fine details about the interface that enables the interaction between proteins. When characterizing PPI … 展开

There are a multitude of methods to detect them. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. The most conventional and widely used high-throughput methods are yeast two-hybrid screening 展开